Abstract:Sesamum (
Sesamum indicum) known variously as sesame, til, gingelly, simsin, gergelin etc, belong to family pedaliaceae. In view Sesamum is rich source of oil content (40-50%), seed protein (20%). Infected seeds carry seed-borne mico-organisms that may cause seed degradation, reduced seed germination, seed vigour and weakening of the plant, resulting in a reduction in plant population. Therefore it is necessary to do investigation on seed mycoflora associate with sesamum.
Five varities were tested to detect the seed borne mycoflora by standard blotter paper method, pre treatment blotter method and agar plate method. In that Aspergillus niger is dominant fungi associate with seeds of sesamum. Three fungicides and two botanicals were tested against Aspergillus niger under in vitro condition using poison food technique. In three fungicides carbendazim+mancozeb and tebuconazole completely inhibited the mycelial growth (100%) followed by hexaconazole (79.25%). In botanicals maximum mycelial growth inhibited by Allium sativum (73.46%) than neem leaf extract (62.58%). Four bioagents viz., Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens and Bacillus subtilis were tested against Aspergillus niger by dual culture method. Maximum mycelium growth inhibition was observed in Trichoderma viride (78.20%) followed by Trichoderma harzianum (73.07%) Pseudomas fluorescens a whereas, Bacillus subtilis were less effective exhibited (28.20, 41.66%) against Aspergillus niger.