Vol. 11, Issue 12 (2022)

Author(s):
Pallavi Shelar, Sarang Dilip Pophaly, Manorama, Awadhesh Kumar Tripathi, Shakeel Asgar, Archana Khare, Lata Jain and Virendra Kumar

Abstract:
Postbiotics are the soluble factors either synthesized by cells or part of cellular structure which have certain physiological benefits to the host. In the present study, a previously isolated strain Lactococcus lactis subsp. lactis KD2 showing high antimicrobial activity, was used to prepare two different types of postbiotic formats, heat killed Lactococcus (HKL) and cell free supernatant (CFS). In both the cases the cells were heat killed at tested temperatures in presence of induced osmotic stress by sucrose. The optimum combination of heat and sucrose was then used for preparing both the postbiotic formats. The two postbiotic preparations were then tested for antimicrobial activity against selected pathogens and fungi.