Vol. 11, Issue 12 (2022)

In this study 95 germplasm of rice were evaluated to identify rice cultivars for blast resistance, salinity stress, brown plant hopper resistance, drought resistance and gall midge. DNA was isolated from leaf samples of 95 rice germplasmsby following the protocol of Edwards et al., 1991. Simple sequence repeats (SSR) markers were used for determination of germplasm having characters for biotic and abiotic resistance. Among 17 primers some primers were found which are tightly linked with biotic and abiotic characters. The output of the this experiment is that primer RM8225 linked with blast resistance, RM140 and RM1287 were linked with salinity stress, RM6775 and RM309 linked with brown plant hopper resistance, RM212 and RM201 linked with drought stress and RM22709 linked with gall midge. The output of the study helpful for the breeders to select parents in the breeding programme.