Vol. 11, Issue 12 (2022)

Author(s):
MM Tajpara, DR Patel, PM Makwana, IH Kalyani, DK Parasana and NM Shah

Abstract:
The present study was designed to study isolation of PPR virus from various clinical samples and to observe cytopathic effects of PPR. For this, total 37 representative clinical samples positive for PPRV by S-ELISA were subjected for isolation and propagation of PPR virus in vero cells. Out of 37 S-ELISA positive samples, 31 samples showed CPE in first passage. The characteristic cytopathic effects includes ballooning of cells, aggregation of cells and syncytia formation. The conformation of virus was checked by N gene based reverse transcriptase PCR (RT-PCR).