Abstract:The field experiment conducted at AICRP on IFSRP, Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar, Maharashtra (India) was carried out during the year 2018-19 and 2019-20 to evaluate the effect of green manuring and organic formulations on soil biological properties at different growth stages. The experiment was laid out in split plot design with three replication. There were nine treatment combinations. The three main plot treatments of kharif green mauring crop (viz., G0- no green manure crop (Cultivated fallow), G1- sunnhemp and G2- dhaincha), 3 sub plot treatments of rabi crops (viz., C1- wheat, C2- chickpea and C3- onion) and 3 sub-sub plot treatments of organic formulation (viz., O1- jeevamrut, O2- em solution and O3- vermiwash). The results showed that The residual effect of Dhaincha (G2) recorded significantly maximum soil microbial population of fungi (23.92 and 26.45 cfu x 10-5 g-1 soil) and (29.63 and 35.19 cfu x 10-5 g-1 soil), bacteria (57.29 and 60.69 cfu x 10-7 g-1 soil) and (47.69 and 50.44 cfu s 10-7 g-1 soil), actinomycetes (44.66 and 47.49 cfu x 10-4 g-1 soil) and (41.51 and 43.28 cfu x 10-4 g-1 soil) at flowering and at harvest stage respectively, than rest of the green manuring crop treatments during both the years of investigation. While the application of Jeevamrut @ 500 lit ha-1 recorded significantly maximum soil microbial population of fungi (24.17 and 26.58 cfu x 10-5 g-1 soil) and (30.12 and 35.25 cfu x 10-5 g-1 soil), bacteria (57.90 and 61.05 cfu x 10-7 g-1 soil) and (47.68 and 50.23 cfu x 10-7 g-1 soil), actinomycetes (45.12 and 48.14 cfu x 10-4 g-1 soil) and (41.86 and 44.17 cfu x 10-4 g-1 soil) at flowering and at harvest stage respectively, than rest of the organic formulation treatments during both the years of investigation.