Vol. 11, Issue 12 (2022)

Author(s):
Saima Shafi, Malik Asif Aziz, Shahid Padder, Aamir Hassan Mir, Mushtaq Ahmad Bhat, Zaffar Mahdi Dar, Amjad Masood, Tariq Ah. Shah, Mushtaq Malik, Ayman Azad, Zohra Shabir and Bisma Rashid

Abstract:
The present study was aimed to assess the diversity of rhizospheric micro flora of Phaseolus vulgaris (French yellow) as well as their efficacy as potential bioinoculants in organic agriculture under temperate conditions of Kashmir. Isolation and characterization of rhizobacteria are an effort to determine the ability of root colony bacteria to produce various compounds that can be used for various purposes of bio-fertilizer formulations and microbial-based industrial interests. The morphological characters like colony shape, size, texture, colour was recorded along with cell characters like gram’s staining and endospore staining. Also, cultural characters like citrate utilization, oxidase test, methyl red test and catalase test was also evaluated. Apart from these, biochemical characterization was also done to evaluate the PGP production ability along with antifungal activities like Ammonia Production, ACC deaminase activity, Phosphorous Solubilization, HCN production, Chitinase Activity, Phytopathogen inhibition using dual culture technique against Rhizoctonia solani and Fusarium oxysporum. The characterization revealed a high varsity among the rhizobacterial isolates that were isolated from the Rhizosphere depicting a plethora of diversity among the root Rhizosphere of Phaseolus vulgaris (French Bean).