Abstract:The present investigation in relation to “Assessment of soil fertility status of cotton growing soil in Dhamangaon and Chandur block in Amravati district.” was undertaken during 2020-21. Total 60 i.e. 30 Surface and 30 subsurface soil samples were collected and analysed for their physico-chemical properties, macro nutrient status in soil. The results revealed that, the soils under study were clayey in texture, neutral to slightly alkaline in reaction with no salinity hazard, low to high in organic carbon in surface and subsurface soil (0.25-0.91 and 0.23-0.88 per cent) respectively, calcium carbonate were moderately high to high in category. The soil of Dhamangaon and Chandur block were very low to low in category (113.00-235.00 kg ha-1) and (106.52-223.25 kg ha-1) respectively, for available nitrogen, low to moderately high for phosphorus (13.47-25.10) for Dhamangaon block and medium to moderately high for Chandur block (17.55-22.22). Whereas, soil of Dhamangaon block were high to very high (205.00-400.00) for available potassium and Chandur block were moderately high to very high (280.00-406.00), respectively. The soils of Dhamangaon block were medium to high in available sulphur (11.77-20.10 mg kg-1) and Chandur block were low to moderately high (9.89-18.32 mg kg-1).