Vol. 11, Issue 12 (2022)

Lumpy skin disease is an economically devastating and transboundary disease in bovine. The disease has been emerging into Asia and Pacific regions in recent times. Polymerase chain reaction (PCR) based molecular detection of Lumpy skin disease virus (LSDV) genome is the most appropriate method in terms of time and cost. The present study compares the analytical sensitivity of various PCR assays targeting the LSDV genome using 5 different gene specific primers. Based on their analytical sensitivity or limit of detection, it is recommended that the P32 PCR is the most sensitive one for detection of LSDV genome in field samples.