Vol. 11, Issue 12 (2022)

Author(s):
Mahendra K Chouksey, Tushar J Bedse, Santosh V Sawardekar, RS Deshpande, VV Dalvi, Samiksha R Chavhan, Hemant S Sawant and Govind B Pawde

Abstract:
The most valued table cultivar of mango (Mangifera indica L.), known as "Alphonso," has been grown in India for more than a century. In order to explore intra cultivar heterogeneity based on microsatellite markers, 15 seedlings of Alphonso mango were grown by the stones taken from identified mother plant from Agriculture Research Station, Shirgaon, District-Ratnagiri (Maharashtra). It was investigated that whether there was genetic heterogeneity or relatedness between the chosen seedlings and their mother plant. The all fourteen verified mango-specific simple sequence repeats (SSRs) were found polymorphic. In the result we found total 224 alleles were produced by polymorphic microsatellites, they showed the 100 % polymorphism. The Jaccard’s similarity coefficient ranged from 0.037 to 0.867. The polymorphic information content (PIC) values were observed between 0.218 (SSR-19) to 0.964 (SSR-26). The Microsatellites with a high degree of polymorphism expressed by primer SSR-26, SSR-24, SSR-16 and MngSSR-26, were more effective in differentiating the chosen alphonso samples.