Vol. 11, Issue 12 (2022)

Dibutyl Phthalate (DBP) is one of the commercially used plasticizer globally. The available research on cellular cytotoxicity of DBP in mammalian cells is not completely understood. The present experiments hence examined the consequences of cell viability and cytotoxicity in the DBP induced ovine granulosa cells at various dose levels. Cytotoxic assays: Neutral red uptake (NRU), Lactate dehydrogenase (LDH) and Sulforhodamine B (SRB) assays were employed to measure the cell viability and cytotoxicity. Cytotoxicity (%) measured by NRU in the GCs exposed to various concentrations of DBP was found to be significantly higher at 50µM dose onwards. Cytotoxicity (%) measured by LDH in the GCs exposed to various concentrations of DBP was found to be significantly higher only at 50µM dose specifically. Similarly, cytotoxicity (%) measured by SRB in the GCs exposed to various concentrations of DBP was found to be significantly higher from 10µM dose onwards linearly compared to the control groups respectively. Overall, DBP induce cellular toxicity by targeting not only plasma membrane but also subcelluar targets such as lysosomal membranes.