Vol. 11, Issue 12 (2022)

Tomato is a commercially important crop cultivated across the globe. Numerous studies states regeneration of tomato via the organogenesis pathways with its limitation. It is important to achieve a reliable and fast in-vitro system that can be best suited to the number of tomato varieties. The major goal of this research was to develop an efficient and rapid protocol for in-vitro regeneration in tomato (Arka vikas). The regeneration capability of hypocotyls was studied in Arka vikas commercial variety of tomato. Arka Vikas seeds were surface sterilized and germinated on ½ MS. Two weeks old hypocotyls were used as explants and regenerated on media containing various combinations of BAP, Zeatin, and IAA. Among these MS media with 2mg/l Zeatin + 0.1 mg/l IAA + 400 mg/l Timentin gave the highest shooting and MS +1mg/l IAA gave the highest rooting frequency. Using this aforementioned protocol we got 80-90% regeneration of tomato plants within 60-65 days.