Vol. 7, Issue 3 (2018)

Author(s):
Prachi P Parvatikar and SB Madagi

Abstract:
The objective of the present work was to evaluate in vitro antimicrobial and antioxidant activity of Indigofera hochestetteri. The selected plant was collected in the vicinity of the University campus. Herbal extraction was done with leaf and rhizome of Indigofera hochestetteri by Soxhlet extraction method with use of ethanol as solvent. Phytochemical analysis was done using different biochemical tests. Antimicrobial activity was performed by using standard agar well diffusion method whereas antioxidant potential of plant extract was analyzed by following 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The preliminary phytochemical analysis of ethanolic leaf extract and legume extract of Indigofera hochestetteri contained broad spectrum of secondary metabolites such as flavonoids, saponin, steroids, amino acid and carbohydrate. The extracts from being screened against six microorganism including fugal strain. The antimicrobial screening showed good activity in both extracts against S. aureus, B. Subtillis, E. coli and antifungal activity against A. niger. The both extracts exhibited dose dependant antioxidant activity. Further study requires isolation, Characterization and structural elucidation of specific bioactive compounds in both extracts that may help in the development of new drugs.