Vol. 12, Issue 2 (2023)

Author(s):
Jagadeesh U, Muthuraju R, Nalini BS, Kadalli GG and Yogananda SB

Abstract:
This study was carried out to select the potential combination of lignocellulolytic cultures using rice straw substrate for degradation. Efficient lignocellulolytic cultures were used to assess their degradation potential using paddy straw. On the basis of all the qualitative assays and quantitative assays, the most efficient lignocellulolytic microorganisms were selected for assessment of degradation potential under greenhouse conditions. Four most efficient cultures consisting of two fungi, one bacterium strain and one actinobacterial strain which were identified as Phanerochaete chrysosporium UASBLCF_01, Purpureocillium lilacinum UASBLCF_02, Bacillus inaquosorum UASBLCB_03 and Streptomyces viridosporus UASBLCA_04, were selected for experiment and the isolates were found to be compatible with each other. and exhibited significant paddy straw decomposition. Steady decrease in Neutral Detergent Fiber (NDF) and ADF (Acid Detergent Fiber) was observed instead of rapid decrease. For the first sampling from pots of decomposing paddy straw at 15 DAC, T₁₃ containing paddy straw and all the four cultures showed maximum decrease in NDF to 45.54 percent as compared to 68.81 percent of control and ADF to 30.43 percent as compared to 46.14 percent of control. There was a significant decline in NDF and ADF of paddy straw pre-treated with different combinations of potential lignocellulolytic cultures.