Vol. 7, Issue 4 (2018)

Author(s):
Kolipaka Rajesh, B Swathi, G Aruna Kumari and M Shanmugam

Abstract:
Culture grade oocytes collected from slaughtered healthy buffaloes were randomly allotted to 4 treatment (T) groups containing oocyte maturation media and were allowed to mature at 38.5^oC and 5% CO₂ in humidified atmosphere for 22 hours. Tissue Culture Medium 199 (TCM 199B), supplemented with Gentamicin was used as control (T₁), while for groups T₂, T₃ and T₄ kisspepetin (Kp) was supplemented to control media at 5 µg/ml, 10 µg/ml and 15 µg/ml respectively. The oocytes were evaluated based on the percentages of Cumulus Cell Expansion (CCE) and 1^st Polar Body extrusion (PB). Addition of Kp to the maturation media observed that the proportion of oocytes showing CCE and PB was significantly (P ≤ 0.5) higher when compared to the control. Among all the three concentrations of Kp, the proportion of oocytes showing the percentage of CCE and PB was highest in T₃ (73.21% and 22.11%, respectively) compared to the T₂ and T₄. Inferring that 10 µg/ml concentration of Kp would yield optimum in vitro maturation of buffalo oocytes.