Vol. 12, Issue 3 (2023)

Double infiltration is also one of the technique along with paraffin, paraffin with ceresin and celloidin method. Which has been described in many staining technique text books. The concept of double infiltration by different authors have given a positive method for staining different tissue components as well as better histological sections, however the use of jelly wax along with paraffin as a double infiltration, has not been described in the literature reviewed. In the present study jelly wax was used as a first infiltrating media and paraffin as a second infiltrating media.

Jelly wax is also a type of paraffin with low viscosity. Being lower viscosity it has got a better infiltration property. There by an attempt has been made to study the effect of double infiltration in different organs. Landes et al. (2005) has used double infiltration in plastinated specimens with celloidin infiltrating media and embed in cedax.