Vol. 12, Issue 3 (2023)

Author(s):
Komal Sharma, Khushbu Verma and Ashok Kumar Meena

Abstract:
Fluoride affects plants in negative manner through increasing the level of ROS species and reducing the plant growth. In this study Guar (Cyamopsis tetragonoloba L. Taub) and Moong (Vigna radiata L.) was exposed to fluoride stress in a half-diluted Hoagland solution. A concentration- dependent analysis (Cyamopsis tetragonoloba L. 0, 10, 20, 30, 40, 50, and 60) and (Vigna radiata L. 0, 2.5, 5, 7.5, 10, 12.5, and 15 mM NaF). The study revealed, as a consequence of the high stress produced by fluoride, we observed that both and SOD and CAT gene showed changes in the expression in Guar (Cyamopsis tetragonoloba L.) and Moong (Vigna radiata L.) root treated samples in comparison to the control. However, the up-regulation changes observed in treated root sample is more evident for Catalase generate 30 mM concentration Guar (Cyamopsis tetragonoloba L. Taub.) and for 7.5 mM concentration for Moong (Vigna radiata L.). Contrary to it SOD gene showed down- regulation for either Guar (Cyamopsis tetragonoloba L.) or Moong (Vigna radiata L.) samples at 30 mM and 7.5 mM concentrations respectively. In the fluoride concentration-based analysis, the highest expression of SOD and CAT was observed in the roots compared to the control.