Vol. 12, Issue 3 (2023)

Author(s):
Veena, Pragash RA, Satheesh G, Suresh N Nair, Bibu J Kariyil, Vergis J, Muneer A, Ajith Kumar KG, Ravindran R and Juliet S

Abstract:
In this investigation, meropenem (MEM) in broiler bird’s plasma was determined using a novel, sensitive and selective high-performance liquid chromatographic technique. In the devised procedure, methanol: glacial acetic acid solution mixture was employed as the mobile phase on a C18 column (Enable, 5µ, 250 X 4.60 mm) with a Phenomenex C18 (4.0 × 3.0 mm, 5 μm) guard column at 40 °C. Chromatographic separation was done at a flow rate of 1 mL/min and a diode array detector was used for detection at 300 nm. The analytes were extracted from plasma using the protein precipitation method with orthophosphoric acid in methanol with doripenem used as the internal standard. The calibration curve was linear over the concentration range of 0.1-1 μg/mL with a correlation coefficient of 0.9989. The values for the limits of detection and quantification were discovered to be 0.31 μg/mL and 0.94 μg/mL, respectively. The validated method can be effectively used to study the pharmacokinetic behavior of meropenem in broiler birds.