Vol. 12, Issue 3 (2023)

Author(s):
Aparna Hebbar, Payal Rani, Suneel Kumar Onteru and Dheer Singh

Abstract:
Urine is a non-invasive bio-fluid used for biomarker detection. The present study aims to assess the feasibility of urine collection in buffaloes. Total six healthy buffaloes were observed for urination for seven days. If animal did not urinate within 20 minutes, buffaloes were stimulated for urination by pouring water on rump, tying or offering water. Out of 84 attempts, only 60 times urine was collected, suggesting ~72% chances of urine collection. Time-wise study showed that 4.76% urination was within 0-5 min, 3.57% urination within 5-10 min, 8.33% urination within 10-15 min and 21.42% of urination within 15-20 min. 8.33% buffaloes urinated by pouring water on rump. If animal did not urinate after 20 min, 3.57% buffaloes showed urination when they were offered water and 21.42% urination was seen when animals were tied in the horns. In conclusion, there is nearly 72% feasibility of urine collection for biomarker analysis.