Vol. 12, Issue 3 (2023)

Author(s):
Jagriti Srivastav and RK Dhuria

Abstract:
The present investigation was carried out to evaluate the effects of dietary incorporation of Nano zinc and phytase enzyme on haematological parameters of Kadaknath layers. For the study, four hundred Kadaknath layers of twenty nine weeks of age, were selected and randomly distributed into ten dietary treatment groups having four replicates in each group with ten birds per replicate. The experimental Kadaknath layers were fed on different levels of Nano zinc and Phytase enzyme either alone or in combination for a period of twenty weeks. The dietary regimens for different treatment groups were as follows: T₁(control)- Basal diet, T₂- Basal diet+30 mg/kg Nano zinc, T₃- Basal diet+40 mg/kg Nano zinc, T₄- Basal diet+50 mg/kg Nano zinc, T₅- Basal diet+60 mg/kg Nano zinc, T₆- Basal diet+ 500 FTU/kg phytase enzyme, T₇- Basal diet+30 mg/kg Nano zinc+ 500 FTU/kg Phytase enzyme, T₈- Basal diet+40 mg/kg Nano zinc+ 500 FTU/kg phytase enzyme, T₉- Basal diet+50 mg/kg Nano zinc+ 500 FTU/kg phytase enzyme, T₁₀- Basal diet+60 mg/kg Nano zinc+ 500 FTU/kg phytase enzyme. At the end of the experiment one bird from each replicate was sacrificed and blood samples were collected in EDTA coated vials at the time of slaughter. The samples were analyzed by standard procedures for the estimation of haematological parameters such as haemoglobin, packed cell volume, total erythrocyte count, total leukocyte count and differential leukocyte count. The observations recorded in the present study revealed no significant differences in the haematological parameters of Kadaknath layers as a result of dietary addition of Nano zinc and Phytase enzyme. Thus the study concluded that Nano zinc and Phytase enzyme can be safely incorporated in the diet of Kadaknath layers up to the level of 60 mg/kg and 500 FTU/kg, respectively.