Vol. 7, Issue 4 (2018)

Author(s):
S Svirska and A Grytsyk

Abstract:
The investigation of tannins in Anchusa officinalis L. was conducted. As a result, qualitative and quantitative tannins content in the herba and the roots were determined. For this purpose we have used high-performance liquid chromatography, titrimetric and spectrophotometric methods. We have revealed gallocatechin, epigallocatechin, catechin, epicatechin, epicatechingallate, catechingallate and gallic acid, and determined their quantitative content by the high-performance liquid chromatography. The total tannins content established using titrimetric method is between 3,00 - 3,53%, spectrophotometric method- 1,13 - 1,74%, high-performance liquid chromatography- 4,22-4,29%. The highest content is recorded for epigallocatechin (51, 79 % and 69, 56 % relative to the total tannins content in Anchusa officinalis L.herba and roots respectively).