Vol. 7, Issue 4 (2018)

Author(s):
Versha Suman, Manisha Parmar and Urmila Gupta Phutela

Abstract:
Evaluation of poultry dropping based biodigested slurry as a substrate for cellulolytic enzyme production using Penicillium roqueforti NCIM712 was the main objective of this study. Poultry droppings and poultry dropping based biodigested slurry were examined for cellulolytic enzyme activity. Production of cellulolytic enzymes from poultry dropping based biodigested slurry was estimated. Endoglucanase activity was highest in both unautoclaved and autoclaved biodigested slurry i.e. 60.05 and 43.81 U/ml of BDS respectively. Exoglucanase activities were found to be 21.15 and 9.06 U/ml of BDS in unautoclaved and autoclaved respectively. Out of all the three enzymes of celluase complex, activities of β glucosidase were found to be minimum viz. 4.81 and 2.13 U/ml of BDS in unautoclaved and autoclaved slurry respectively. Protein content was also high in unautoclaved (4.53 mg/ml) than autoclaved (2.13 mg/ml) biodigested slurry. As biodigested slurry is rich in organic matter along with consortium of microorganisms of diverse groups, thus contributing towards higher activities of endoglucanse, exoglucanase and β glucosidase. However after autoclaving, reduction in enzyme activities was reported which might be due to the fact that high temperature (121°C) would degrade and denature some of the proteins and thus affecting catalytic activities of enzyme. These results also suggest that use of unautoclaved slurry for enzyme production will save the cost of autoclaving which is energy consuming process.