Vol. 12, Issue 3 (2023)

Author(s):
M Sonali, N Karuna Sree, YN Reddy, K Dhanalakshmi, MR Reddy and E Kumar

Abstract:
The present study was under taken with a view to characterize BTV 2, 9 and 15 isolates employed for trial vaccine. BTV 2, 9 and 15 virus isolates were cultivated in BHK₂₁cell line and RNA was isolated. Further, RT-PCR was standardized for detection of NS1 gene of BTV 2, 9 and 15. Molecular characterization of these serotypes were taken up by the sequencing of NS1 gene. The sequences were compared to the available sequences in Genbank. Further analysis indicated that all NS1 nucleotide sequences segregated into 6 phylogenetic clades. The BTV-15 (N12) is closely related to BTV-15 (N15), BTV-15 (DQ399835). BTV-2 (M11) and BTV (N12) are clustered together with BTV-9 and BTVTPT, BTV-9 and BTV KMTAI. The identity of BTV-2 (M11) with USA and Taiwan isolates of BTV-2 was 95% and 90%, the identity of BTV -15 with USA isolates of BTV 2 and 17 was 93% and 98% and with BTV Taiwan isolate was 87%. Further analysis indicated that BTV serotype isolates were closely related to BTVTPT.