Vol. 12, Issue 3 (2023)

Author(s):
Sonu Sharma, RK Pandya, Pramod Kumar Fatehpuria, Smita Prajpati and HK Trivedi

Abstract:
Sclerotinia stem rot incited by Sclerotinia sclerotiorum is a serious threat to oilseed production in Northern of Madhya Pradesh. The present investigation was conducted at Department of Plant pathology, College of Agriculture, Gwalior, (M.P.) with ten solid culture media viz., French bean seed agar, Lettuce leaf agar, Pea seed agar, Mustard stem agar, Mustard seed agar, Mustard leaf agar, Mustard stem extract 10%+ sucrose 2% agar, Mustard seed extract 10%+ sucrose 2% agar, Glucose sodium chloride agar and Potato dextrose agar medium were evaluated against S. sclerotiorum. The data revealed that the maximum growth was recorded in Potato dextrose agar (85.33 mm) followed by glucose sodium chloride agar (82.33 mm), mustard leaf agar (72.67 mm) and mustard seed extract 10%+ sucrose 2% agar (69.67 mm), while minimum growth was recorded in lettuce leaf agar (44.33 mm) followed by French bean seed agar (51.67 mm) and mustard stem agar (53.33 mm) respectively. The maximum number of sclerotic per plate was recorded in Potato dextrose agar (10.33/plate) while the minimum number of sclerotic per plate was recorded in lettuce leaf agar (3.67/plate). The maximum size of sclerotic was recorded in Potato dextrose agar (4.67 mm) while the minimum size of sclerotic was recorded in lettuce leaf agar (2.67 mm). The highest weight of sclerotic per plate was recorded in Potato dextrose agar (725.00 mg) while lowest weight of sclerotic per plate was recorded in lettuce leaf agar (230.00 mg). Among culture media, PDA medium was found as most suitable media for mycelia growth and sclerotic formation of the Sclerotinia sclerotiorum.