Vol. 12, Issue 4 (2023)

Author(s):
Sabhyata Sharma, Anish Yadav, Vikas Yadav, Harsh Sharma, Swapnil Magar and Nitin Mishra

Abstract:
Babesia canis vogeli and Ehrlichia canis, are two canine haemoprotozoans transmitted by ticks, Rhipicephalus sanguineus in India. The present report deals with the standardisation and diagnosis of Babesia canis vogeli and Ehrlichia canis infection in dogs suspected with babesiosis and ehrlichiosis. The canines showing symptoms of high fever, anorexia, debility, anaemia, hind limb swelling were selected and diagnosed using duplex PCR for simultaneous detection of Babesia canis vogeli and Ehrlichia canis, using species specific primers. The results confirmed the standardisation of duplex PCR for simultaneous detection of Babesia canis vogeli and Ehrlichia canis.