Vol. 12, Issue 4 (2023)

Author(s):
Analisha Debbarma, Sidhartha Sankar Behera, Indramani Nath, Smruti Ranjan Mishra, Susen Kumar Panda and Ritun Patra

Abstract:
The study was conducted on Swiss albino mice to determine the efficacy of adipose-derived mesenchyme stem cells (AD-MSCs) on wounds along with collagen granules. The adipose tissues were collected from the abdominal cavity and processed following standard procedure, and then seeded in a six well cell culture plate, which was incubated at 37 ºC in a humidified CO₂ (5%) incubator till 70–80% confluence. Then the cells were injected subcutaneously along the margin of the wound, and collagen granules were applied in addition. On average, 13.3±0.33 days were required for complete healing, and the wound contraction area was 3.21±0.04 mm, 1.88±0.02 mm, and 1.14±0.05 mm on days 3^rd, 7th, and 14th, respectively. Wounds heal faster because of their individual properties. And this combination showed a promising usefulness in treating wounds by inducing the healing process.