Vol. 12, Issue 4 (2023)

Author(s):
Nishiswapna Garnayak, Santosh Kumar Senapti, Ramesh Chandra Patra, Susen Kumar Panda, Geeta Rani Jena and Smruti Ranjan Mishra

Abstract:
Development of antibiotic resistance among most common pathogens has put the light on use of plant and their parts as an alternative therapy option. Andrographis paniculata is one of the most used plants as documented by many researchers having antibacterial potential. The present study was done to evaluate its leaves extract efficacy against Escherichia coli, Staphylococcus aureus. Aqueous, methanolic and ethanolic leaf extracts were prepared by soxhlet method and used at 500mg/ ml, 750mg/ml and 1000mg/ml concentration in antibacterial test discs (6mm). The extracts were evaluated for antibacterial activity by disc diffusion method in quintuplicate number. No zone of inhibition for could be observed in case of aqueous extracts, while ethanolic extract (@ 1000mg/ml) showed a zone of inhibition (Mean ± SD) of 6.16 ± 0.114 mm & 6.98 ± 0.130 mm against Escherichia coli, Staphylococcus aureus with percentages of inhibitions 61.97% and 70.08%. The methanolic extract (@ 750mg/ml and 1000mg/ml) showed zone of inhibition amounting 6.16 ± 0.089, 7.1 ± 0.070 and 7.08 ± 0.083, 7.82 ± 0.130 mm against Escherichia coli, Staphylococcus aureus with percentages of inhibitions 61.97%, 71.42% and 71.08%, 78.51% respectively. Methanolic extracts were found to be most effective against both the bacteria.