Vol. 12, Issue 4 (2023)

Author(s):
Varsha Paladan, Vinod Kumar Chaturvedi and Bablu Kumar

Abstract:
A pilot approach to explore the strain interference between two strains of Brucella abortus were attempted. Two common laboratory adapted strains of B. abortus, strain19 and strain 99 which could be differentiated based on their metabolic requirements and antibiotic susceptibility were selected and their interactions were studied. Experiments were made by direct inoculation of the selected strains in Brucella broth and to understand any interference of one strain on other. The interference or interaction between the selected strains was evaluated by co-cultivation and pre-culture techniques under varying conditions. The study results suggest that there is no significant difference in viable count if both the strains were inoculated together in to broth or when the S99 was inoculated into a 3h growth of S19, except for a 1-log (log₁₀) enhancement. At the same time, the growth of S99 got inhibited when S19 was inoculated 3h after the S99 inoculation into the same broth. In conclusion, there is some in vitro interference of S19 on S99 strains, suggesting that they could be promising candidates for protection against brucellosis if supporting evidences unveiled. The clinical significance of the obtained results must be judged with caution as the interference studies were performed in vitro and only two bacteria were used. The present research approach can be considered as one among the pilot studies, further studies are warranted to evaluate the preventive effect of S19 against S99 and to suggest the possible therapeutic use if feasible.