Vol. 12, Issue 4 (2023)

Author(s):
Nilesh S Chavan, Kshama G Deshpande, Pandharinath H Janjal, Parimal R Udgave and Kundan R Jadhao

Abstract:
This Experiment was carried out in Department of Biotechnology at Mahatma Gandhi Mission College of Agricultural Biotechnology, Gandheli. The experiment was carried out in year 2014-15. The objective of the study was to establish suitable protocol for synthesis of silver nanoparticles using biological method. Different plant extracts were prepared and tested for their ability to synthesis nanoparticles. Biological mean of nanoparticle synthesis is easy, cost effective and eco-friendly. The experiment was laid out in factorial randomized block design (FRBD) with 65 treatment combinations of 13 different plants and 5 concentration of silver nitrate solution (1 mM, 2 mM, 3 mM, 4 mM and 5 mM). Each plant extract was added to different concentrations of silver nitrate solution in separate test tube. Formation of silver nanoparticles were primarily confirmed by color change followed by UV-VIS spectrophotometer. Among all the 65 treatments 13 were found positive for synthesis of silver nanoparticles. The UV-VIS readings are referred with the previous research paper readings. This study clearly indicate that synthesis silver nanoparticle by using plant extract is easily possible.