Vol. 12, Issue 4 (2023)

Author(s):
Nilesh S Chavan, Kundan R Jadhao, Sonam S Kale, Pandharinath H Janjal and Akash T Shinde

Abstract:
This in-vitro study gives us natural antibacterial agents which can beuseful to increase the efficacy of antibiotics The experiment was carried out at MGM College of Agricultural Biotechnology, Gandheli during year 2018-19. The objective of the study was to find out suitable combination of medicinal plant extract and antibiotics. Five different medicinal plants Zingiber officinale, Opuntia ficus indica, Bryophyllum pinnatum, Syzygium aromaticumand Curcuma longawere selected for this study on the basis of their medicinal properties. The methanolic extract of these plant were mixed with solution of four different anibiotics. Total twenty treatment combinations were tested against three different bacterial pathogens Escherichia coli,Pseudomonas syringaeand Xanthomonas campestris. These combinations were tested against pathogens using agar well diffusion assay. The experiment was carried out in Factorial Randomized Block Design (FRBD).The zone of inhibition was measured for each combination and compared with zone of inhibition of individual plant extract and antibiotics. It was observed that combination of Opuntia ficus indica + Gentamicin showed highest the zone of inhibition 38 mm compared to all other combinations. Plant extracts have great potential as antimicrobial compounds against microorganisms. Thus, they can be used in the treatment of infectious diseases caused by resistant microbes.