Vol. 12, Issue 4 (2023)

Author(s):
Sonam S Kale, Narendra R Chavan, Ganesh V Kore and Kundan R Jadhao

Abstract:
Trichoderma has gained immense importance since last few decades due to its biological control activity against several plant pathogens. In the present investigation five isolates of Trichoderma were collected from different geographical locations of Gandheli, Aurangabad region and characterized primarily on the basis of morphological characters. Molecular characterization of isolates of Trichoderma revealed homogeneity with RAPD primer. The genetic relatedness among five isolates of Trichoderma was analyzed by using six RAPD markers. In total 22 alleles were detected in five Trichoderma isolates. The number of alleles per loci ranged from 3 to 2 with an average of 2.6 alleles per locus (Table.6). All the RAPD loci show polymorphism. Analysis of genetic diversity among five isolates revealed that the polymorphic Information Content (PIC) value represents the relative informativeness and in this study, the average PIC value was found to be 0.44. A PIC value was found to be ranged from 0.26 to 0.56. Expected heterozygosity or Gene diversity computed according to Nei (1973) and varied from 0.32 to 0.64 with an average of 0.52.