Vol. 12, Issue 4 (2023)

Author(s):
Nagajothi S, Mekala P, Raja A, Raja MJ, Senthil Kumar P and Thirumalaisamy G

Abstract:
Newcastle disease remains a constant threat to poultry sector especially backyard chicken due to practical difficulties in following the vaccination protocol. Andrographis paniculata was reported to possess antiviral activity against several viruses affecting human and animal. The present study was designed to investigate the antiviral activity of Andrographis paniculata against mesogenic strain of Newcastle disease virus NDV2K35. Antiviral activity of aqueous, ethanolic extracts of Andrographis paniculata, andrographolide standard each @1.0, 2.5, 5 µg/ml were compared with standard antiviral drug ribavirin @ 500 µg/ml in chicken embryo fibroblast cells. The antiviral activity was confirmed by assessing the cell viability, HA titre and expression of F gene by RT-PCR in cell culture fluid. Aqueous extract and andrographolide standard exhibited antiviral activity at all doses and was on par with standard antiviral drug ribavirin. The ethanolic extract was unable to completely inhibit the virus which was evident from HA titre and expression of F gene.