Vol. 12, Issue 4 (2023)

Author(s):
Sahil Kumar, Vijay Kumar, Indu and Richa

Abstract:
In the present study estimation of microbial diversity in skin normal microflora is done. Total 8 isolates were purified from 2 samples (OW1, OW2, OW3 & OW4) (FW1, FW2, FW3 & FW4) on the basis of colony morphology. In office worker the shape of colony were observed Spindle (OW1 & OW3) and two isolates (OW2 & OW4) were irregular. In office worker the colony pigmentation two isolates (OW2, OW3) were whitish and (OW1) are yellow and OW4 are creamy yellow in colony pigmentation. In colony elevation two isolates (OW1, OW3) were flat and (OW2) isolate were raised (OW4) were filamentous in colony elevation. In colony margin two isolates (OW1, OW4) are entire and (OW2) isolate were crenate, and (OW3) were observed lobate in colony margin. In Field worker the shape of colony were observed three isolates are irregular (FW1, FW2 & FW3) and one isolate (FW4) were rhizoidal in colony shape. In Field worker the colony pigmentation were observed two isolates (FW2, FW4) are creamy and One isolate (FW1) are whitish and (FW3) are yellowish in colony pigmentation. In colony elevation (FW1) isolate were flat, (FW2) isolate were raised, (FW2) isolate were convex & (FW4) were filamentous in colony elevation. In colony margin two isolates (FW1, FW2) are entire and (FW4) isolate were filamentous, and (FW3) isolate lobate were observed in colony margin. Total 8 isolates were purified from 2 samples namely, office work, (OW1, OW2, OW3 & OW4) field work, (FW1, FW2, FW3 & FW4) From office worker palm’s skin (OW1, OW3) isolates were negative &(OW2, OW4) were positive for gram staining and in field worker palm’s skin (FW1, FW3) isolates were negative & (FW2, FW4) were positive for gram staining. Total 8 isolates were purified from 2 samples namely, office work, (OW1, OW2, OW3 & OW4) field work, (FW1, FW2, FW3 & FW4), From office worker palm’s skin (OW1, OW2, OW3 & OW4) all the 4 isolates were positive for catalase test. From field worker palm’s skin (FW1, FW2, FW3 & FW4) all the 4 isolates were positive for catalase test.