Vol. 12, Issue 4 (2023)

Author(s):
M Roshni, R Ambily, M Mini, BK Mani, T Naicy and PS Reshma

Abstract:
The present study involves molecular diagnosis of leptospirosis in a cat. A tomcat aged one year was presented to the clinics with symptoms of anorexia and vomiting. Blood (in EDTA) and serum samples were submitted to the Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Kerala, suspecting leptospirosis. The serum sample was subjected to Microscopic Agglutination Test (MAT) employing a panel of 12 live reference serovars of Leptospira as antigen. The sample was found to be negative. Blood sample was subjected to Polymerase Chain Reaction (PCR) targeting loa22 gene of Leptospira and amplicon of 525 bp was obtained, thus confirming it as a case of leptospirosis.