Vol. 12, Issue 4 (2023)

Author(s):
Raj Verma, Abhishek Verma and Savarni Tripathi

Abstract:
Crape jasmine(Tabernaemontana divaricata) exhibiting stem fasciation symptom was observed in November 2022 at Indian Agricultural Research Institute, Regional Station campus in Pune (Maharashtra) India. As fasciation is a typical symptom induced due to phytoplasma infection in plants, PCR and nested PCR using phytoplasma-specific primer pairs was carried out to determine the possible association of a phytoplasma with the symptomatic sample. Amplification was not obtained in the symptomatic sample during direct PCR with two sets of primers and also in the nested PCR assay. This is the first report of occurrence of stem fasciation in T. divaricata and the observed symptom is not associated with a phytoplasma infection.