Vol. 12, Issue 5 (2023)

Author(s):
Sagar SP, Mallesha BC, Vajramma B and Akshay Mamidi

Abstract:
The bacterial endophytes are present within the host plants and confer beneficial effects in plants. In the present study, 12 bacterial endophytes were isolated from Leucas aspera and Mimosa pudica. Further, were characterized based on colony morphology and biochemical tests. Bacterial endophytes colony morphology varied with different coloration, margin, elevation, and translucency. Of 12 endophytic bacterial isolates, 33.33% were Gram-positive and 66.66% were Gram-negative with shapes varied between cocci and rod. Fifty percent of the isolates were endospore formers. Biochemical tests for the isolates found positive results for methyl red (33.33%), Voges-Proskaeur (75%), H₂S production (41.67%), carbohydrate fermentation (Glucose, 75%; Sucrose, 70.83%), catalase (83.33%), indole production (54.17%), and citrate utilization (50%). The present study explored the diversity of the bacterial endophytes of Leucas aspera and Mimosa pudica species and has led to the creation of their biochemical profiles.