Vol. 12, Issue 5 (2023)

Author(s):
Sandeep Kumar Sangwan, Surander Singh Dhaka and Ankit Magotra

Abstract:
The present investigation undertaken to study the genetic variability in the Pituitary specific transcription factor-1 gene's polymorphism and its association with performance traits was carried out using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) and nucleotide sequencing techniques. DNA was isolated from 50 Murrah buffalo maintained at LUVAS, Hisar. A 600 bp fragment of the Pit-1 gene was amplified and digested using HinfI restriction enzymes. The genotypes were then sequenced using the Sanger di-deoxy chain termination method and were compared with related species. HinfI locus showed only one genotype. The amplicon of the gene exhibited the absence of polymorphism with respect to restriction enzymes used; accordingly, the allelic frequency was found to be unity. The monomorphic pattern of the amplicon of these genes concerning different enzymes indicated the concernedness of these genes. The result suggested that the allele has been fixed in the herd and needs the inclusion of the Murrah buffalo from other populations to introduce desired genetic variability to enhance milk production.