Vol. 12, Issue 5 (2023)

Author(s):
Dr. Priyanshi Yadav

Abstract:
Over the past century, the study of cell culture has proliferated, leading to the development of several viral vaccine production. Since cell culture technology was first developed, the primary media GMEM is used for BHK 21 cells for vaccine production. BHK 21 cells are anchorage-dependent cells that can be grown or cultured on the polystyrene-based surface and mainly depend on the environment of the media required. Along with serum, the pH of the medium is generally essential for cell cultures. The balance between sodium bicarbonate (NaHCO₃) in the culture medium and CO₂ in the incubator typically maintains the pH of the medium for cell cultures. In this study, the different pH media namely acidic (pH-6.5), normal (pH 7.2-7.4), and alkali (pH-8) were examined for their effect on cell growth.