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Vol. 12, Issue 5 (2023)

The use of polymerase chain reaction assay for the detection of bovine herpes virus-1 infection (BHV-1) in cattle in organized and un-organized farms

Author(s):
Keneisezo Kuotsu, N Bhumapati Devi, Sashitola Ozukum, Laltlankimi, Neithono Kuotsu and Tukheswar Chutia
Abstract:
This research study was carried out to detect the antigen prevalence of Bovine Herpes Virus-1 (BHV-1) infection among two groups of cattle, which were maintained and reared in organized farms and in un-organized farms. A total number of 107 serum sample were collected randomly from both the farms, comprising of 54 serum samples (n=54) from the organized farms and 53 serum samples (n=53) among un-organized farms, The genomic DNA extraction from the serum samples were carried out for the detection of Bovine Herpes Virus-1 infection by Polymerase Chain Reaction (PCR) using gI gene specific. PCR detected 25 samples from organized farms and 24 samples from un-organized farms, the percentage prevalence of Bovine Herpes Virus-1 infection in organized farms was 47.16 percent (25/53) and 44.44 percent (24/54) in the un-organized farms. The present study emphasizes the diagnosis of BHV-1 infection in Cattle in organized farms and un-organized farms based on Polymerase Chain Reaction (PCR) result.
Pages: 2042-2044  |  132 Views  64 Downloads


The Pharma Innovation Journal
How to cite this article:
Keneisezo Kuotsu, N Bhumapati Devi, Sashitola Ozukum, Laltlankimi, Neithono Kuotsu, Tukheswar Chutia. The use of polymerase chain reaction assay for the detection of bovine herpes virus-1 infection (BHV-1) in cattle in organized and un-organized farms. Pharma Innovation 2023;12(5):2042-2044.

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