Vol. 12, Issue 5 (2023)

Author(s):
Biswadeep Behera, Ravindran R, Parthasarathi Behera, Tushar Kanta Garnaik, Biswaranjan Sahoo and Madhusmita Mohanta

Abstract:
Pneumonic Pasteurellosis, also known as respiratory mannheimiosis, is the most common infection having wide prevalence in ruminants. The disease is caused by P. multocida, Gram negative, non-motile, non-spore forming, facultative anaerobic, small rods or coccobacilli commensal in upper respiratory tract and gastrointestinal tract. During the study period, total 95 number of tissue samples were collected from slaughtered animals. Out of these, 30 swabs were collected in culture broth from suspected lungs samples. The tissue samples suspected for Pasteurellosis were collected in 10% NBF for fixation and then histopathological analysis was performed. Microscopic study revealed that the affected lungs were dominated by interstitial and alveolar oedema along with vascular thrombosis of capillaries and small blood vessels. And characteristic oat shape cells. The intestine and other visceral organs like liver, kidney and spleen showed moderate to severe haemorrhages. 7 samples were found positive for Pasteurella sp. expressing hyaD-hyaC gene with amplification at 1044 bp size.