Vol. 12, Issue 5 (2023)

Author(s):
Dr. Biswadeep Behera, Dr. Ravindran R, Dr. Parthasarathi Behera, Dr. Biswaranjan Sahoo, Dr. Tushar Kanta Garnaik and Dr. Madhusmita Mohanta

Abstract:
Bovine babesiosis is a one of the serious threat to cattle industry due to treatment and tick control costs, production loses and animal mortality. It is mainly caused by Babesia bovis and Babesia bigemina in the tropics and subtropics. Slaughterhouse was regularly visited and blood samples were randomly collected in EDTA vials from bovines being slaughtered, for the study of different hemoparasitic infections. Total 100 number of bovine blood samples were collected properly in EDTA vials from Govt. slaughterhouse Mizoram. Thin blood smears were prepared and stained with 10% Giemsa stain and observed under oil immersion lens (100 X magnifications) for presence of Babesia sp. Further blood samples were undergone Molecular detection through PCR analysis. Total 22 number of samples were found positive out of 100 no. of blood samples in morphological diagnosis whereas 27 samples were found positive for Babesia bigemina in molecular diagnosis using a portion of the mitochondrial DNA of Babesia bigemina. The incidence of Babesia sp. infections was more common in summer followed by winter and monsoon.