Vol. 12, Issue 5 (2023)

Author(s):
Preeti Toppo, GL Sharma, Prabhakar Singh, Hement Panigrahi and LS Verma

Abstract:
The present investigation entitled “Rapid in-vitro propagation of strawberry cv. Nabila” which was carried out during the year 2018-19 and 2019-20 at Commercial Tissue Culture Laboratory Department of PMBB, College of Agriculture, IGKV, Raipur (C.G.) G.) Hence, a study was conducted to standardize stage wise in-vitro media for in-vitro rapid multiplication of strawberry cv. Nabila and to find out best combination of plant growth regulators for various stages of in-vitro multiplication experiments with 3 replications and 24 treatments combinations. Experiment is laid out in Completely Randomized Design. The present study demonstrates the combined effects various growth regulators viz., BAP, IBA, NAA, Kinetin and GA₃ at varied concentration with MS medium on various shoot prolifaction, multiplication and rooting media, . In shoot prolifaction media, MS medium supplemented with BAP (0.1 mg/l) and NAA (0.1 mg/l) resulted minimum number of days required for culture establishment and MS + Kinetin (1.0 mg /1) + NAA (0.5 mg/1) recorded maximum number of shoots/ clumps per explant. In shoot multiplication media, MS + Kinetin (1.0 mg /1) + NAA (0.5 mg/1) recorded maximum number of shoots/ clumps per explant. The maximum length of shoot of strawberry explant was observed with (MS + Kinetin (1.0 mg /1) + NAA (0.1 mg/1) and MS + BAP (0.1 mg /1) + NAA (0.1 mg/1) recorded minimum days to shoot. In shoot elongation and rooting media, minimum days required to root induction on strawberry explant was observed with MS+ NAA (0.50 mg/l) + Activated Charcoal (200 mg/l) and MS medium supplemented with NAA (1.00 mg/l) + Activated Charcoal (200 mg/l) was found to be the most effective and resulted higher roots and when coming to rooting percentage MS medium with NAA (1.00 mg/l) emerged as best during both the years of study (2018-19 and 2019-20).