Vol. 12, Issue 5 (2023)

Author(s):
Tarundeep Singh, HR Bhardwaj, Hansmeet Kour and Zul I Huma

Abstract:
The current investigation was carried out in cattle (n=30), that underwent laparo-enterectomies, to assess the organisms responsible for causing surgical site infection and devise measures to control the post-operative infection. Each case was followed-up for 35 days post-operatively on alternative days for checking the surgical site infection. The pus sample in positive cases was collected aseptically and sent for the identification of bacteria using culture and various biochemical tests. The antimicrobial susceptibility test was also done to know the level of resistance among various bacteria and use an appropriate antibiotic for the treatment. Surgical site infections (SSIs) occurred in 12/30 cases. The mean duration of diagnosis of SSI was found to be 8.08±0.93 days. Staphylococcus, Streptococcus, and E.coli were the organisms isolated. Among these, Staphylococcus aureus was the commonest species isolated accounting for 83.4% of the SSIs as single colonies in 6 cases and mixed colonies in 4 cases. Bacterial isolates of Staphylococcus aureus and Streptococcus spp. showed 100% sensitivity against gentamicin and enrofloxacin, followed by 66.6% each to ceftriaxone and penicillin. Isolates of E. coli showed maximum sensitivity (100%) against enrofloxacin and gentamicin. Consistent surgical management of the site and the specific antibiotic regimen used were effective in treating post-operative SSIs without further complications.