Vol. 12, Issue 6 (2023)

Seabuckthorn (Hippophae rhamnoides L) has recently gained interest for its nutritional and medicinal values. Fruits and leaves are considered to be good source of large number of bioactive substances such as vitamins, trace elements, amino acids, β-carotene, zeaxanthin, lycopene, flavonoids, folic acid, fatty acid, tannic acid etc. Chemoprofiling (Total phenols, total flavonoids, vitamin C, vitamin E, lycopene and β-carotene contents) of various seabuckthorn byproducts has been done and it was found that among all the seabuckthorn byproducts, leaf extract contained significantly highest amounts of total phenols (332.49±7.45 mg/g), total flavonoids (271.56±5.41 mg/g), vitamin C (399.49±4.90 mg/100g) and lycopene content (8.50±2.92 mg/100g).

The proximate analysis of nutritive contents of Seabuckthorn byproduct was determined. The ash content which is an index of mineral contents, ranged from 1.3 to 4%. The moisture content was significantly highest in seedcake. The crude protein contents ranged from 13.89 to 23% and recorded highest in leaves (22.09%) and seedcake (23%). Ether and crude fibre contents were recorded highest in pomace with seeds. High concentrations of sodium (Na) were present, ranging from 40 to 160 mg/g. Among all the byproducts; leaves contained high concentration of all the minerals estimated.

Seabuckthorn byproducts were screened for the presence of antioxidant potential for inhibiting the different in vitro free radicals. The inhibition of the free radicals i.e. ABTS, DPPH, superoxide, hydroxyl and nitric oxide radicals by all the byproducts was found in concentration dependent manner. The IC50 values for different radicals were determined and from the IC50 values, it was observed that among the seabuckthorn byproducts, leaves had lowest IC50 value for all the free radicals and was better scavenger of these radicals. The reducing power of the extracts was also in dose dependent manner. The leaves showed better reducing power ability as compared to other extracts.