Vol. 12, Issue 6 (2023)

Author(s):
CM Bhaliya, HJ Kapadiya, JG Chavda, DS Kelaiya and RB Thanki

Abstract:
Chickpea (Cicer arietinum L.) is the second most important pulse crop grown in tropical, subtropical and temperate regions of the world. Among the different soil borne diseases, wilt and root rot complex caused by Fusarium oxysporum f. sp. ciceris, F. solani and Macrophomina phaseolina is the most important, devastating and challenging disease of chickpea. For the screening of any plants against any pathogens, a suitable inoculation method with appropriate inoculum density is essential. In present investigation, effect of different inoculation methods (soil inoculation and seed inoculation) on pathogenicity of F. oxysporum f. sp. ciceris, F. solani and M. phaseolina on chickpea were evaluated. All fungal pathogen isolate produce typical wilt and root rot complex disease symptoms in chickpea. The soil inoculation method with cent percent disease incidence was remain highly effective and the most suitable method to prove the pathogenicity of F. oxysporum f. sp. ciceris, F. solani and M. phaseolina in chickpea. In case of seed inoculation method, all the fungal pathogens exhibited drooping and yellowing of leaves followed by bark shredding and rooting of root in chickpea. F. oxysporum f. sp. ciceris was remain the most virulent pathogen among all the tested pathogen in seed inoculation method with 82.50 per cent disease incidence followed by F. solani (70.00%) and M. phaseolina (62.50%).