Vol. 12, Issue 6 (2023)

Author(s):
Pallvi Slathia, Deepti Narang, Mudit Chandra, Sikh Tejinder Singh and Maroudam Veeraswamy

Abstract:
A total of 20 blood samples were collected from bTB reactor animals (n=10) and bTB non-reactor animals (n=10) collected from cattle and buffaloes to study the cytokine gene expression of IFN- γ, TNF- α and IL-4 in both the bTB reactor group of animals and the non-reactor group of animals. Real time PCR was used to study the gene expression in in vitro DST (MPB83,70) stimulated PBMCs. IL-4 gene expression was seen only in 4 animals. Ct value of IL-4 ranged from 32 to 34 (in bTB reactor animals) and >36 (in non-reactor animals). When RQ of these samples was calculated it showed an upregulation in one animal (RQ 1.380) while it was down regulated in three animals (RQ 0.0268, 0.128 and 0.752) when compared with bTB negative animal. TNF-α gene expression was seen only in 3 animals. Ct value of TNF-α ranged from 30 to 33 (in bTB reactor animals) and >34 (in non-reactor animals). Upregulation was observed in only one sample (RQ 2.702) and down regulation was observed in two samples (0.266 and 0.213) when compared to non-reactor group of animals based on their RQ value. IFN- expression gene expression was seen in 5 animals. Ct value of IFN-γ ranged from 30 to 35 (in bTB reactor animals) and >37 (in non-reactor animals). IFN-γ expression was found to be upregulated in 4 bTB reactor animals (RQ 6.294, 1.142, 3.92 and 1.034) and downregulated in one animal (RQ 0.22) based on RQ value.