Vol. 12, Issue 6 (2023)

Author(s):
Chandrashekara Naik, Bijurkar RG, Venkanagouda D, Tandle MK, Suranagi MD and Shrikant Kulkarni

Abstract:
This study was conducted to compare the effect of L-ascorbic acid and goat follicular fluid supplementation on in-vitro maturation of goat oocytes. In order to evaluate the effect of these two supplements, cumulus oocytes complexes (COCs) were collected from goat ovaries by aspiration, slicing and puncture method. Irrespective of oocyte retrieval techniques, grade-I oocytes (n=337) in 16 replicates were matured in-vitro in TCM-199 (with estradiol @ 1 µg/ml, eCG @ 20 IU/ml, hCG @ 20 IU/ml, 10% EGS (Estrus goat serum) and Gentamicin sulphate @ 50 µg/ml) as control that was supplemented with 50 µg/ml of L-ascorbic acid and 20% goat follicular fluid for 27 hours. Assessment of maturation was done based on the degree of cumulus cells expansion and extrusion of first polar body under stereo-zoom microscope. Significantly higher (p<0.05) proportion of oocytes were matured in-vitro in medium supplemented with goat follicular fluid compared to medium supplemented with L-ascorbic acid and control. It was concluded that enriching the maturation medium with goat follicular fluid has beneficial effect on in-vitro maturation of goat oocytes.