Vol. 12, Issue 6 (2023)

Author(s):
Venkata Yaswanth Amara, JJ Dhruv, Akarsh Parihar, Parthavi Patel and Nirali Gamit

Abstract:
Potato (Solanum tuberosum L.) is the fourth most important food crop in the world and an important vegetable crop. Use of molecular markers to determine genetic variation, genetic diversity and evolutionary relatedness is becoming more popular for the assessment of diversity among cultivars. EST-SSR amplification of 42 potato cultivars using 61 EST-SSR primers generates 4382 scorable bands with an average of 72 bands per primer. The grouping provided by cluster analysis showed cultivar identification and divergence among potato cultivars. All 42 cultivars were classified into 5 main clusters where Kufri Nilkanth, 20-(P-42-600), 17-(P-35-400), Kufri Khyati, Kufri Kesar, Kufri Ashoka, 19-(P-40-500), 4-(C-20-200), and 1-(C-10-400) most diverge one. Genotypes could be identified based on specific banding pattern. Inter Simple sequence repeat (ISSR) analysis showed ISSR amplification of 42 potato cultivars using 29 ISSR primers generates 6002 scorable bands with an average 207 bands per primer. The cluster analysis showed identification of cultivar and variance among 42 cultivars, grouped the cultivars into 4 clusters where Kufri Himalini, 28-(RH-2-600), Kufri Chandramukhi, 20-(P-43-200) and 26-(P-54-300).