Vol. 7, Issue 5 (2018)

Author(s):
Sridevi B, Viroji Rao ST, Gnana Prakash M, Ravinder Reddy V and Krishna D

Abstract:
Genetic characterization of IWD and IWF strains of White Leghorn birds was undertaken based on analysis of 10 microsatellite markers that involved 120 birds. The distribution of microsatellite markers in each of these strains helped decipher genetic heterogeneity, population genetic structure and evolutionary relationships. All the microsatellite loci utilized for the analysis were polymorphic and reasonably informative; overall 4 primers (ADL176, MCW0014, ADL0210 and ADL158) showed PIC more than 0.5 which are very informative markers. Among the 5 loci the highest (4) number of alleles were obtained for loci ADL176, ADL0210, and the lowest (2) at the locus MCW0110. However the loci MCW0014 and ADL158 revealed 3 alleles in two strains studied. The number of alleles ranged from 2 to 4 in both strains, observed heterozygosity ranged from 0.23 (ADL158) to 0.40 (ADL210) in IWF and 0.22 (ADL 158) to 0.40 (MCW014) in IWD.