Vol. 7, Issue 5 (2018)

Author(s):
MS Kala, LN Sankhala, M Champawat, SR Puniya and NM Shah

Abstract:
The application of multiple serological assays currently available for the detection of Brucella antibodies in various species of animals indicates that no single test can detect all infected animals and therefore, combination of serological tests should include more sensitive tests designed to reduce the number of false negative reactions which contribute to the persistence of infection as a herd problem. So the presnt study was conducted to evaluate the comparative efficacy of RBPT and STAT to i-ELISA determined with regards to their sensitivity and specificity in the diagnosis of bovine brucellosis for detecting antibodies in cows and buffaloes. In present study seropositivity for Brucella antibodies were detected by i-ELISA, RBPT and STAT in 618 serum samples of cattle and buffaloes, comprising of 296 from cattle and 322 from buffaloes. Of these 12.46%, 20.06% and 35.92% of serum samples were found to be positive by RBPT, STAT and i-ELISA,, respectively showing higher efficacy i-ELISA followed STAT and RBPT.The sensitivity of RBPT and STAT was found to be of 34.68% and 55.86%, respectively, considering i-ELISA as a gold standard test while specificity was found to be of 98.48% and 98.99%, respectively. Thus, STAT was found to be more sensitive and specific than that of RBPT.