Vol. 12, Issue 6 (2023)

Author(s):
Sujata, Anil Kumar, Vinod Kumar and Lochan Sharma

Abstract:
Leachates of four organic amendments were collected and tested in vitro, for their effect on the encumbrance of endospore of Pasteuria penetrans to second stage juvenile (J₂) of Meloidogyne javanica. Among the tested organic amendments, higher attachment of endospores was observed in leachates collected from mustard cake and minimum was observed from farmyard manure. Then, application of P. penetrans at different spore load (1x10³, 1x10⁴ and 1x10⁵ spores/g soil) in combination with mustard cake at the rate of 10 g/kg soil was tested for the management of M. javanica in tomato nursery. All the three doses of P. penetrans along with mustard cake improved plant growth parameters. Maximum seedling height and seedling weight were observed in treatment where P. penetrans at the rate of 1x10⁵ spores/g soil was applied. The number of galls per root system and reproduction potential of root-knot nematode decreased with the increase in the dose of bacterial parasite. Minimum number of galls, eggs per root system and final nematode population were observed, where parasitic preparation of P. penetrans was applied at dose of 1x10⁵ spores/g soil along with mustard cake.